Whole Genome Sequencing
Whole Genome Sequencing
Whole-genome sequencing (WGS) is a comprehensive method for analyzing entire genomes. Genomic information has been instrumental in identifying inherited disorders, characterizing the mutations that drive cancer progression, and tracking disease outbreaks. Rapidly dropping sequencing costs and the ability to produce large volumes of data with today’s sequencers make whole-genome sequencing a powerful tool for genomics research.
Whole Genome Sequencing Introduction
Aruna Genomics’ human whole genome sequencing (WGS) service detects the complete genome sequence at one time and provides a high-resolution, base-by-base view of the genome. This enables researchers to see both large and small variants and identify potential causative variants for further follow-on gene expression or regulation mechanism studies.
WGS can be applied to a wide range of human genetics research applications including, evolution studies to detect genome-wide genetic variations and pathogenic and susceptibility gene analysis. It can also be applied to translational research to provide information on cancer and disease-associated mutations, and is one of the most important research approaches in precision medicine.
Our high quality, cost-efficient WGS services have seen most of our partners transition away from microarray-based genotyping studies. We provide WGS services in a range of coverages to suit all research requirements. Contact us for a no obligation discussion about your project.
Sample Requirements
Regular Samples:
PCR Library
• ≥ 200ng (Recommend ≥ 400ng)
• ≥ 8ng/μL
PCR-free Library
• ≥ 1μg (Recommend ≥ 2μg)
• ≥ 12.5ng/μL
Low Input Samples
• ≥ 50ng
• ≥ 2.5ng/μL
Sequencing Standards
• PE100 or PE150
• Guaranteed 85% of bases with quality score of Q30
• Standard sequencing coverage 30X/90Gb data (60X is recommended for cancer samples)