Small RNA Sequencing
Small RNA sequencing is used to discover novel small RNAs, examine the differential expression of all small RNAs and to characterize variations with single-base resolution. Contact us today with any questions or for a no obligation quote.
Small RNA Sequencing Introduction
Small RNAs are a type of non-coding RNA (ncRNA) molecule that are less than 200nt in length. They are often involved in gene silencing and post- transcriptional regulation of gene expression. Our sequencing scale and extensive experience ensure high quality, fast small RNA servcies at unbeatable pricing.
Discover Our Unique Molecular Identifiers (UMIs) Service
Unique Molecular Identifiers (UMIs) can be utilized to eliminate undesirable PCR duplicates derived from a single molecule. After PCR, molecules sharing a UMI are assumed to be derived from the same input molecule. As such, UMI counts offer superior results to counting reads, leading to more accurate estimates of quantitative small RNA expression. UMI technology is especially beneficial to customers undertaking research on rare and precious samples or samples containing less RNAs, such as exosomes.
Sample Requirements
Regular Samples:
• Mass: ≥ 1 μg
• Concentration: ≥ 50 ng/μl
• Volume: 15 μl
General Quality Requirements:
• RIN ≥ 6.0 (Plant/Animals)
(Note, we can process degraded samples at lower quality thresholds. Contact us for more information.)
Sequencing Standards
• Guaranteed ≥85% of bases with quality score of ≥Q30
• ≥20 million reads per sample is recommended