16S/18S/ITS Sequencing

16S/18S/ITS Sequencing

We provide professional, fast and affordable 16S/18S/ITS gene sequencing services for the identification, classification and quantitation of microbes within complex biological mixtures. Contact us today with any questions or for a no obligation quote.

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16S/18S/ITS Introduction

16S and 18S rDNA are hypervariable regions in the 16S or 18S rRNA genes in bacteria and fungus, while ITS (Internal Transcribed Spacer) is the spacer DNA between the small-subunit and large-subunit rRNA genes in bacteria, fungi and archaea.

Sequence comparison of 16S/18S/ITS regions is widely used in taxonomy and molecular phylogenetics because these regions have a high degree of variation even between closely related species, while also offering easy amplification by PCR, even from low quantities of DNA.

We have extensive experience supporting partners across the world with their 16S, 18S rDNA and ITS research needs, while our economies of sequencing scale and proprietary technology ensures extremely efficient pricing.

Sample Requirements

Genomic DNA

  • Mass ≥ 50ng
  • Concentration ≥ 6ng/μL

PCR Product:

  • Mass ≥ 3μg
  • Concentration ≥ 10ng/μL

Sequencing Standards

  • Up to ≥ 80% of bases with a ≥ Q30 quality score, depending on the chosen sequencing strategy
  • Recommended sequencing coverage is dependent on the complexity of the sample