RNA Sequencing (Transcriptome)
Our purposeful technology yet highly affordable animal and plant whole genome sequencing services have been relied upon by researchers acOur world leading RNA Sequencing services are relied upon by researchers and companies all over the world. Our scale and experience enable us to offer industry beating pricing, highly efficient turnaround times and fantastic project support. Contact us today with any questions or for a no obligation quote.ross the world. Contact us today with any questions or for a no obligation quote.
RNA Sequencing Introduction
RNA Sequencing, sometimes referred to as transcriptome sequencing, is used to reveal the presence, quantity and structure of RNA in a biological sample under specific conditions. Compared to hybridization-based RNA quantification methods such as microarray analysis, sequencing-based transcriptome detection can quantify gene expression with low background, high accuracy and high levels of reproducibility within a large dynamic range. In addition, transcriptome sequencing does not require an existing genome sequence and can detect splice variants and fusion genes that can not be detected by microarrays.
With over two decades of experience, we have supported most of the world’s leading research institutes and pharma companies as well as many thousands of other partners across the globe. Our experience, sequencing scale and proprietary technology enables quality RNA data, rapidly delivered, all at industry beating pricing. Contact us today for a no obligation quote.
Sample Requirements
Human/Mice/Rat (non-whole blood)
Total RNA: ≥ 200ng,
Concentration: ≥ 10ng/μl
General Quality Requirements:
RIN: ≥ 7.0
28S/18S: ≥ 1.0
(Note, we can process degraded samples at lower quality thresholds. Contact us for more information.)
Sequencing Standards
• Guaranteed ≥ 80% of bases with quality score of ≥ Q30
• 100bp and 150bp paired-end sequencing options available
• ≥30 Million reads per sample recommended