Post-Translational Modification (PTM) Analysis
Post-Translational Modification (PTM) Analysis
Post-translational modification (PTM) analysis reveals the functional state and regulatory dynamics of proteins by mapping modifications such as phosphorylation, glycosylation, and acetylation. Because PTMs dictate protein function, localization, and interaction networks, high-resolution PTM mapping provides key functional context beyond simple protein abundance.
PTM Analysis Introduction
Aruna’s PTM analysis platform uses specialized affinity enrichment technologies combined with high-resolution mass spectrometry to localize and quantify modified amino acid residues across the proteome. Our deep phosphoproteomics and glycoproteomics pipelines enable researchers to trace signal transduction cascades, enzyme activation states, and immune receptor modifications with site-specific accuracy.
PTM profiling is critical in kinase inhibitor screening, cell signaling research, oncology functional genomics, and biotherapeutic characterization. Mapping modification sites provides an operational understanding of how extracellular stimuli translate into cellular action.
Our robust enrichment protocols ensure high selectivity for low-abundance modified peptides, overcoming interference from unmodified background proteins. We provide comprehensive site-localization reports with statistical localization probability scores. Contact us for a no-obligation discussion about your project.
Sample Requirements
Enrichment Input (Phospho / Glyco):
Cell Pellets: ≥1×107 cells (treated/stimulated)
Tissue Input: ≥100 mg (snap-frozen immediately upon harvest)
Extracted Protein: ≥500μg to 1mg total protein (Phospho-enrichment requires higher starting input)
Sample Preparation & Inhibitors:
Phosphatase/Protease Inhibitors: Samples must be harvested in the presence of strong phosphatase/protease inhibitor cocktails.
Purity: Strictly free from detergent and nucleic acid contamination.
Technical Standards
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TiO2 / Fe-NTA magnetic bead enrichment for phosphopeptides; Lectin / HILIC enrichment for glycopeptides
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Guaranteed modification site-localization probability ≥99% (Class I site assignment)
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Simultaneous profiling of global protein expression alongside PTM enrichment cohorts