Targeted Proteomics (MRM / PRM)

Targeted Proteomics (MRM / PRM)

Targeted proteomics using Multiple Reaction Monitoring (MRM) and Parallel Reaction Monitoring (PRM) offers gold-standard sensitivity, specificity, and dynamic range for quantitating predefined candidate protein panels. This approach provides an antibody-independent alternative for validating biomarker candidates across large clinical cohorts with absolute precision.

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Targeted Proteomics Introduction

Aruna’s targeted proteomics service delivers hypothesis-driven quantification for high-priority protein sets. Utilizing synthetic isotope-labeled standard (SIS) peptides, our PRM and MRM assays provide precise concentration measurements for low-abundance targets, even within highly complex matrices such as human plasma, cerebrospinal fluid, and cell lysates.

Targeted proteomics is essential for candidate biomarker validation, clinical translation, pathway verification, and verifying findings derived from broader discovery proteomics studies. It bypasses the cross-reactivity issues commonly associated with antibody assays while maintaining exceptional quantitative precision.

Our targeted assay development team assists with peptide selection, transition optimization, and standard curve generation. Whether validating a 10-protein signature or monitoring a 100-peptide panel, we customize every assay parameter to your research requirements. Contact us for a no-obligation discussion about your project.

Sample Requirements

Biological Matrices:

  • Plasma / Serum: ≥20 μL

  • Biofluids (CSF, Urine, Saliva): ≥100 μL

  • Cell/Tissue Lysate: ≥20 μg total protein

Pre-Digested Peptides / Custom Inputs:

  • Peptide Input: ≥5-10 μg clean peptide mixture

  • SIS Peptides: Optional (Provided by user or synthesized custom by Aruna)

  • Buffer Compatibility: Volatile buffers preferred (0.1% Formic Acid / ACN)

Technical Standards

  • Triple Quadrupole (MRM) or High-Resolution Q-Exactive/Orbitrap (PRM)

  • Linear dynamic range spanning up to 105 with isotope-dilution standards

  • Guaranteed quantitative precision with CV<10% across clinical validation cohorts