Single Cell Sequencing

Single Cell Sequencing

Single cell sequencing is used for the study of heterogeneous samples, rare cell types, cell lineage relationships, and disease evolution. We offer high quality, affordable single cell services, with world-class support from our highly experienced single cell project experts.

Quick Quote

Single Cell Sequencing Introduction

Single cell sequencing is commonly applied to solve problems associated with the study of heterogeneous samples, rare cell types, cell lineage relationships, and disease evolution. By using deep sequencing of DNA and RNA from a single cell, cellular functions of individual cells can be investigated in great detail.

Aruna applies the superior method of Multiple Displacement Amplification (MDA) for single cell genomic DNA amplification (WGS/WES), and Switching Mechanism at 5′ End of RNA Template (SMART-Seq2) for single cell RNA amplification (transcriptome/RNA quantification). Contact us with your project requirements, our experts are here to help.

Sample Requirements

DNA

Single Cell: 1-2 cells – 4 µL PBS buffer

A Few Cells: 2-1000 cells – 4 µL PBS buffer

Amplified single cell gDNA/cDNA:

Concentration: >20 ng/µL, Quantity: >3 μg

RNA

Single Cell: 1-2 cells – 4 µL lysis buffer

A Few Cells: 2-1000 cells – 4 µL lysis buffer

Amplified single cell gDNA/cDNA:

Concentration: >0.2 ng/µL, Quantity: >1 ng

Total RNA for low input library:

Concentration: >50 pg/µL, Quantity: >2 ng;

RNA 28S/18S ≥1，RIN ≥7

Sequencing Standards

  • PE100 or PE150 sequencing option, depending on your application.
  • Guaranteed ≥85% of bases with a quality score of ≥Q30.
  • Cell sorting by mouth-controlled microcapillary pipetting system.