Immune Repertoire Sequencing

Aruna provides advanced NGS based Immune Repertoire Sequencing services to amplify and sequence the immune repertoire to help researchers evaluate the diversity of immune repertoire cost-effectively and precisely.

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Immune Repertoire Sequencing Introduction

Traditional strategies for studying the immune repertoire, including spectating and Sanger sequencing, are expensive, time-consuming, and insufficient for generating a high-resolution picture of the immune repertoire.

Aruna’s Immune Repertoire Sequencing (IR-SEQ) service enables partners to examine the adaptive immune system with bias-controlled multiplex PCR or 5’ RACE amplification and high-throughput sequencing of T-cell and B-cell receptors.

IR-Seq allows researchers to better understand adaptive immune-mediated diseases and supports research into a wide range of other applications.

Sample Requirements

Sorted T cells RNA/PBMCs RNA

Library Type: Mul-PCR or 5’RACE

Volume 15 μl – 100 μl

Mass ≥ 0.5 μg

Concentration ≥ 35 ng/ μl

RIN ≥ 7.0

28S/18S ≥ 1.0

Sequencing Standards

• HiSeq: PE100/150 for mul-PCR library

• MiSeq: PE300 for 5’RACE library

• Mul-PCR: short insert library

• 5’RACE: long insert library