Whole Genome Re-sequencing

ANIMAL AND PLANT WHOLE GENOME SEQUENCING​

Our purposeful technology yet highly affordable animal and plant whole genome sequencing services have been relied upon by researchers across the world. Contact us today with any questions or for a no obligation quote.

Animal and Plant Whole Genome Sequencing Introduction

Aruna Genomics’ animal and plant whole genome sequencing, also referred to as whole genome re-sequencing (WGRS) involves sequencing the entire genome of a animal or plant, and comparing the sequence to that of a known reference genome. Re-sequencing of the animal and plant genome will identify genetic variations such as SNPs and InDels, and is often applied for the identification of functional genes and markers of important traits to facilitate molecular breeding and to improve agricultural production and conservation.

WGS provides a more comprehensive, accurate and efficient tool for animal and plant genomics projects that traditionally have used legacy technology such as genotyping arrays or genotyping-by-sequencing. The WGS approach outperforms these methods by providing an order of magnitude more data, greater statistical power, and enhanced variant discovery capabilities, easier analysis – all at a lower cost. 

Our end-to-end workflow can support you with a broad range of species. We also evaluate and accept custom projects on a case-by-case basis. We can perform deep sequencing for a select number of individuals and build a well assembled reference genome for imputations.

Sample Requirements

Regular Samples: 

PCR

– Mass: ≥200 ng (Recommend ≥400 ng)
– Concentration: ≥8 ng/μL

PCR-free

– Mass: ≥1 μg (Recommend ≥2μg)

– Concentration: ≥12.5 ng/μL

Low Input Samples: 

PCR

– Mass: ≥50 ng 
– Concentration: ≥2.5 ng/μl

Animal/Plant Whole Genome Sequencing Service Overview

• PCR and PCR-Free library methods both available 

• 100bp and 150bp paired-end sequencing available

• Guaranteed ≥85% of bases with a quality score of Q30

• Standard sequencing coverage 5 – 10X for population studies 

• Standard sequencing coverage 10 – 30X for individual study 

• Typical 10 working days from sample QC acceptance to filtered raw data availability, depending on sequencing system.

• Expedited services are available, contact us for more detail.

• Reports and output data files are delivered in industry standard file formats: BAM, .xls, .png and FASTQ data.